In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets
In vitro differentiation of naïve CD4+ T cells into effector and regulatory subsets offers a means to acquire large numbers of relatively homogeneous cell populations for experimentation. However, culture systems for T cell differentiation described in the literature vary widely in efficiency and complexity, limiting their comparison across studies. Here, we present a standardized and robust method for the isolation and in vitro differentiation of six CD4+ T cell subsets from mouse naïve T cells.
Key wordsCD4 T cells In vitro differentiation Polarization Helper T cells Cytokines Th1 Th2 Th17 iTreg
This work was supported by NIH grant R01 GM115474 to M.C.