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Detection, Expansion, and Isolation of Human MAIT Cells

  • Yu Liu
  • Wei Wang
  • Xiongwen Wu
  • Xiufang WengEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2111)

Abstract

Mucosal-associated invariant T (MAIT) cells are a novel subset of innate-like T cells that recognize vitamin B metabolites from a range of microbes presented by MHC class I-related molecules (MR1). The term mucosal-associated invariant T cells derives from the fact that MAIT cells are abundant in the liver and mucosal tissues, and human MAIT cells use a semi-invariant TCR Vα7.2 Jα33 paired with Vβ2 or Vβ13. Here, based on the interaction between MAIT cell and its ligand 5-OP-RU/MR1, we describe the protocols for identification, rapid expansion, and isolation of human MAIT cells.

Key words

MAIT cells MR1 5-OP-RU Semi-invariant TCR 

References

  1. 1.
    Tilloy F An invariant T cell receptor a chain defines a Novel TAP-independent major histocompatibility complex class Ib-restricted a/b T cell subpopulation in mammals. J Exp Med 189(12):1907–1921CrossRefGoogle Scholar
  2. 2.
    Reantragoon R, Kjer-Nielsen L, Patel O, Chen Z, Illing PT, Bhati M et al (2012) Structural insight into MR1-mediated recognition of the mucosal associated invariant T cell receptor. J Exp Med 209(4):761–774CrossRefGoogle Scholar
  3. 3.
    Martin E, Treiner E, Duban L et al (2009) Stepwise development of MAIT cells in mouse and human. PLoS Biol 7(3):e54CrossRefGoogle Scholar
  4. 4.
    Treiner E, Duban L, Bahram S (2003) Selection of evolutionarily conserved mucosal-associated invariant T cells by MR1. Nature 422:164–168CrossRefGoogle Scholar
  5. 5.
    Dusseaux M, Martin E, Serriari N et al (2011) Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood 117:1250–1259CrossRefGoogle Scholar
  6. 6.
    Huang S, Gilfillan S, Kim S, Thompson B, Wang X, Sant AJ et al (2008) MR1 uses an endocytic pathway to activate mucosal-associated invariant T cells. J Exp Med 205(5):1201–1211CrossRefGoogle Scholar
  7. 7.
    Le Bourhis L, Mburu YK, Lantz O (2013) MAIT cells, surveyors of a new class of antigen: development and functions. Curr Opin Immunol 25(2):174–180CrossRefGoogle Scholar
  8. 8.
    Kjer-Nielsen L, Patel O, Corbett AJ, Le Nours J, Meehan B, Liu L et al (2012) MR1 presents microbial vitamin B metabolites to MAIT cells. Nature 491(7426):717–723CrossRefGoogle Scholar
  9. 9.
    Maggi L, Santarlasci V, Capone M, Peired A, Frosali F, Crome SQ et al (2010) CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC. Eur J Immunol 40(8):2174–2181CrossRefGoogle Scholar
  10. 10.
    Le Bourhis L, Martin E, Péguillet I, Guihot A, Froux N, Coré M et al (2010) Antimicrobial activity of mucosal-associated invariant T cells. Nat Immunol 11(8):701–708CrossRefGoogle Scholar
  11. 11.
    Marrack P, Gold MC, Cerri S, Smyk-Pearson S, Cansler ME, Vogt TM et al (2010) Human mucosal associated invariant T cells detect bacterially infected cells. PLoS Biol 8(6):e1000407CrossRefGoogle Scholar
  12. 12.
    van Wilgenburg B, Scherwitzl I, Hutchinson EC, Leng T, Kurioka A, Kulicke C et al (2016) MAIT cells are activated during human viral infections. Nat Commun 7:11653CrossRefGoogle Scholar
  13. 13.
    Won EJ, Ju JK, Cho YN et al (2016) Clinical relevance of circulating mucosal-associated invariant T cell levels and their anti-cancer activity in patients with mucosal-associated cancer. Oncotarget 7:76274–76290CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Immunology, School of Basic Medicine, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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