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Artificial Antigen Presentosomes for T Cell Activation

  • Yi-Geng Pang
  • Chien-Chung ChangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2111)

Abstract

CD8+ T cells constitute an essential component of the adaptive immune system. They are activated through T cell receptor (TCR) recognizing antigenic peptides presented by MHC class I molecules expressed by antigen-presenting cells, such as dendritic cells (DCs). Harvesting a large number of activated, antigen-specific human CD8+ T cells for functional studies has been a laborious task for immunologists, largely because of the variables associated with DC preparations. Here we describe a robust, cost-effective DC-free antigen-presenting system capable of generating a large number of antigen-specific CD8+ T cells in vitro.

Key words

T cell activation Major histocompatibility complex Human leukocyte antigen Antigen presentation 

Notes

Acknowledgments

This work was supported in part by the National Science Council grant (NSC 98-2320-B-007-004-MY3) and the Ministry of Science and Technology grants (MOST 106-2320-B-007-001; MOST 107-2320-B-007-002) to C.-C. C.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute of Molecular and Cellular BiologyNational Tsing Hua UniversityHsinchuTaiwan

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