Artificial Antigen Presentosomes for T Cell Activation
CD8+ T cells constitute an essential component of the adaptive immune system. They are activated through T cell receptor (TCR) recognizing antigenic peptides presented by MHC class I molecules expressed by antigen-presenting cells, such as dendritic cells (DCs). Harvesting a large number of activated, antigen-specific human CD8+ T cells for functional studies has been a laborious task for immunologists, largely because of the variables associated with DC preparations. Here we describe a robust, cost-effective DC-free antigen-presenting system capable of generating a large number of antigen-specific CD8+ T cells in vitro.
Key wordsT cell activation Major histocompatibility complex Human leukocyte antigen Antigen presentation
This work was supported in part by the National Science Council grant (NSC 98-2320-B-007-004-MY3) and the Ministry of Science and Technology grants (MOST 106-2320-B-007-001; MOST 107-2320-B-007-002) to C.-C. C.
- 3.Chen L, Flies DB (2013) Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol 13:221–242Google Scholar
- 12.Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H et al (2000) Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta2-microglobulin-associated and beta2-microglobulin-free HLA class I heavy chains. J Immunol 165:3275–3283CrossRefGoogle Scholar