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Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation

  • Yang Zhang
  • Xuehui Long
  • Xiaoming WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2111)

Abstract

Follicular helper T (Tfh) cells constitute a specialized CD4+ T-cell subset that localizes in close proximity to B cells and are essential in the production of high-affinity, class-switched antibodies, and their dysregulations are also involved in autoimmune diseases. Modulating gene expression patterns in primary T cells is an important approach to understanding Tfh cell differentiation and function. In this chapter, we describe a protocol to evaluate Tfh cell differentiation with OT-II TCR transgenic T cells by retrovirally transducing gene of interest. This protocol adopts the recombinant retrovirus-based transduction of primary CD4+ T cells, and it also includes procedures for adoptive transfer, immunization, and flow cytometry analysis.

Key words

Retroviral vector Spinfection Follicular helper T cells Adoptive transfer OVA-Alum Flow cytometry 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of ImmunologyNanjing Medical UniversityNanjingChina

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