Interleukin-8-Induced Invasion Assay in Triple-Negative Breast Cancer Cells

  • Mohammad M. Uddin
  • Bijaya Gaire
  • Betsy Deza
  • Ivana VancurovaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


The pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8) induces proliferation and invasion of solid tumor cells. In many types of solid cancer, including triple-negative breast cancer (TNBC), the IL-8 expression is induced by proteasome inhibition. In this chapter, we describe a protocol for the analysis of TNBC cell invasion induced by IL-8 in response to proteasome inhibition by bortezomib (BZ). Using this approach, we show that BZ increases the invasion ability of TNBC cells, and that the BZ-increased TNBC cell invasion is suppressed by IκB kinase (IKK) inhibition, which also decreases the IL-8 expression. The experimental protocol includes the cell invasion assay, microscopic evaluation of the invading cells, and quantitative analysis of the obtained images. This protocol should be applicable also for measurement of chemokine-induced tumor cell invasion in other types of cancer cells.

Key words

Bortezomib Cancer cell invasion Chemokines Interleukin-8 IκB kinase Proteasome inhibition Triple-negative breast cancer 



This work was supported by NIH grant CA202775 to I. Vancurova.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Mohammad M. Uddin
    • 1
  • Bijaya Gaire
    • 1
  • Betsy Deza
    • 1
  • Ivana Vancurova
    • 1
    Email author
  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA

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