Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence

  • Herui Wang
  • Ryan L. Pangilinan
  • Yan ZhuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ.

Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primary antibodies from the same host species. Those applications allow detection of cellular proteins with enhanced sensitivity and add flexibility on primary antibody selection in double- or multicolor immunofluorescence staining assays.

Key words

Tyramide signal amplification Immunofluorescence Double-color immunostaining Interleukin-17 receptor B 



This work was supported by St. John’s University and NIH grant CA213426 to Yan Zhu.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biological SciencesSt. John’s UniversityJamaicaUSA
  2. 2.Department of Biological SciencesSt. John’s UniversityQueensUSA

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