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Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma

  • Takaaki KamataniEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Abstract

This section describes an immunohistochemistry method to analyze interleukin-1 (IL-1) in oral squamous cell carcinoma. The described protocol has been optimized for IL-1 detection in formalin-fixed, paraffin-embedded oral tissue sections by light microscopy. A few common pitfalls and problems associated with immunohistochemical staining are discussed.

Key words

Immunohistochemistry Interleukin-1 Oral squamous cell carcinoma Formalin fixation Paraffin embedding 

References

  1. 1.
    Jensen LE (2010) Targeting the IL-1 family members in skin inflammation. Curr Opin Investig Drugs 11:1211–1220PubMedPubMedCentralGoogle Scholar
  2. 2.
    Murzin AG, Lesk AM, Chothia C (1992) beta-Trefoil fold. Patterns of structure and sequence in the Kunitz inhibitors interleukins-1 beta and 1 alpha and fibroblast growth factors. J Mol Biol 223:531–543CrossRefGoogle Scholar
  3. 3.
    Sims JE, Gayle MA, Slack JL et al (1993) Interleukin 1 signaling occurs exclusively via the type I receptor. Proc Natl Acad Sci U S A 90:6155–6159CrossRefGoogle Scholar
  4. 4.
    Dinarello CA (1994) The interleukin-1 family: 10 years of discovery. FASEB J 8:1314–1325CrossRefGoogle Scholar
  5. 5.
    Kamatani T, Shiogama S, Yoshihama Y et al (2013) Interleukin-1 beta in unstimulated whole saliva is a potential biomarker for oral squamous cell carcinoma. Cytokine 64:497–502CrossRefGoogle Scholar
  6. 6.
    March CJ, Mosley B, Larsen A et al (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315:641–647CrossRefGoogle Scholar
  7. 7.
    Matos LL, Trufelli DC, de Matos MG et al (2010) Immunohistochemistry as an important tool in biomarkers detection and clinical practice. Biomark Insights 5:9–20CrossRefGoogle Scholar
  8. 8.
    Coons AH, Creech HJ, Jones RN (1941) Immunological properties of an antibody containing a fluorescent group. Exp Biol Med 47:200–202CrossRefGoogle Scholar
  9. 9.
    Mason DY, Sammons R (1978) Alkaline phosphatase and peroxidase for double immunoenzymatic labeling of cellular constituents. J Clin Pathol 31:454–460CrossRefGoogle Scholar
  10. 10.
    Vacca LL, Hewett D, Woodson G (1978) A comparison of methods using diaminobenzidine (DAB) to localize peroxidases in erythrocytes, neutrophils, and peroxidase-antiperoxidase complex. Stain Technol 53:331–336CrossRefGoogle Scholar
  11. 11.
    Leong AS, Wright J (1987) The contribution of immunohistochemical staining in tumour diagnosis. Histopathology 11:1295–1305CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Oral and Maxillofacial SurgeryShowa University School of DentistryOta-CityJapan

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