Application of Immunohistochemistry in Basic and Clinical Studies

  • Aihua LiEmail author
  • Dong-Hua YangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


Immunohistochemistry (IHC), also known as immunohistochemical staining, is an immune morphological analysis. It is a process of selectively identifying antigens (proteins) by antibodies in cells or tissue sections. This chapter introduces the procedure and application of immunohistochemistry. Although immunohistochemistry has a vast application in basic and clinical studies, this chapter focuses on its application in biomarker study, particularly in biomarkers that related to cancer diagnosis, prognosis, and drug development. Detail protocol of immunohistochemistry in formalin-fixed and paraffin-embedded tissue sections is included.

Key words

Immunohistochemistry Immunofluorescence Biomarkers Diagnostics Cancer treatment 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Epitomics—An Abcam CompanyBurlingameUSA
  2. 2.Department of Pharmacology, College of Pharmacy and Health SciencesSt. John’s UniversityQueensUSA

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