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Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes

  • Kiersten P. Carter
  • Jeffrey E. Segall
  • Dianne CoxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Abstract

Macrophages are known to play multiple roles in the breast cancer microenvironment including the promotion of tumor cell invasion that is dependent on soluble factors or through direct contact. Macrophages can also enhance the production of Tunneling Nanotubes (TNTs) in tumor cells which can be mimicked using macrophage-conditioned medium. TNTs are long thin F-actin structures that connect two or more cells together that have been found in many different cell types including macrophages and tumor cells and have been implicated in enhancing tumor cells functions, such as invasion. Here we describe basic procedures used to stimulate tumor cell TNT formation through macrophage-conditioned medium along with methods for quantifying TNTs.

Key words

Tunneling nanotubes Macrophages Tumor cell Microscopy Conditioned medium 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Kiersten P. Carter
    • 1
  • Jeffrey E. Segall
    • 1
    • 2
  • Dianne Cox
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Anatomy and Structural BiologyAlbert Einstein College of MedicineBronxUSA
  2. 2.Gruss-Lipper Biophotonics CenterAlbert Einstein College of MedicineBronxUSA
  3. 3.Department of Molecular and Developmental BiologyAlbert Einstein College of MedicineBronxUSA

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