Analysis of Circulating HMGB1 in Human Serum

  • Weiqiang Chen
  • Guoqiang Bao
  • Lin Zhao
  • Haichao WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


As a ubiquitous nuclear protein, high-mobility group box 1 (HMGB1) is constitutively expressed and can be actively secreted by macrophages/monocytes, as well as passively released from damaged cells following pathological injuries. Studies indicate that HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Although intracellular HMGB1 functions as a regulator of tumorigenesis, epigenetic anticancer agents or therapeutic γ-ray irradiation could also cause active secretion or passive release of HMGB1, enabling serum HMGB1 to serve as a biomarker for the diagnosis and therapy of various cancers. Here we describe a semiquantitative immune blotting method to measure HMGB1 in human serum, in comparison with a commercially available HMGB1 enzyme-linked immunosorbent assay (ELISA) technique.

Key words

HMGB1 Western blotting ELISA Serum Antibody 



Work in the authors’ laboratory was supported by grants from the National Institutes of Health (R01GM063075 and R01AT005076).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Weiqiang Chen
    • 1
  • Guoqiang Bao
    • 1
  • Lin Zhao
    • 1
  • Haichao Wang
    • 1
    • 2
    • 3
    Email author
  1. 1.Laboratory of Emergency MedicineThe Feinstein Institute for Medical ResearchManhassetUSA
  2. 2.Donald and Barbara Zucker School of Medicine at Hofstra/NorthwellHempsteadUSA
  3. 3.The Feinstein Institute for Medical ResearchNorthwell Health SystemManhassetUSA

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