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Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells

  • Pritpal Kaur
  • Shreya Nagar
  • Madhura Bhagwat
  • Mohammad M. Uddin
  • Yan Zhu
  • Ales VancuraEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Abstract

Interferon-γ (IFNγ) is a pleiotropic cytokine that signals to many different cell types. IFNγ has both antitumor functions and pro-tumorigenic effects and regulates different aspects of cell physiology, including metabolism. Cancer cells undergo a complex rearrangement of metabolic pathways that allows them to satisfy the needs of increased proliferation, and many cancer cells redirect glucose metabolism from oxidative phosphorylation to aerobic glycolysis. In this chapter, we describe a protocol that utilizes the Agilent Seahorse XFp Analyzer to assess mitochondrial respiration and glycolysis in ovarian cancer cells treated with IFNγ.

Key words

Oxygen consumption rate Extracellular acidification Mitochondrial respiration Glycolysis Interferon-γ Ovarian cancer 

Notes

Acknowledgment

This work was supported by NIH grant GM120710 to A. Vancura.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Pritpal Kaur
    • 1
  • Shreya Nagar
    • 1
  • Madhura Bhagwat
    • 1
  • Mohammad M. Uddin
    • 1
  • Yan Zhu
    • 1
  • Ales Vancura
    • 1
    Email author
  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA

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