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Detection of Ferroptosis by BODIPY™ 581/591 C11

  • Alejandra M. Martinez
  • Ahryun Kim
  • Wan Seok YangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Abstract

Ferroptosis is a distinctive form of regulated cell death that is driven by lethal accumulation of lipid peroxides in plasma membranes. Failure to control ferroptosis has been implicated in multiple pathological conditions including cancer development, neurodegeneration, renal injury, ischemia/reperfusion injury, and T-cell immunity. Here we describe a method to detect ferroptosis by determining the amount of lipid peroxides in cellular membranes using BODIPY-C11 probe and flow cytometry. Putative role of ferroptosis in immune modulatory cells can be determined using the same method.

Key words

Ferroptosis Lipid peroxides BODIPY-C11 Erastin Flow cytometry 

Notes

Acknowledgements

This work was supported by National Institute of Health Grants 1SC2NS104275 to W.S.Y.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alejandra M. Martinez
    • 1
  • Ahryun Kim
    • 1
  • Wan Seok Yang
    • 1
    Email author
  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA

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