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Methods for Target Enrichment Sequencing via Probe Capture in Legumes

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Legume Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2107))

Abstract

Target enrichment sequencing (TES) is a powerful approach to deep-sequencing the exome or genomic regions of interest with great depth. Although successfully and widely adopted in many plant species, TES is currently applied for genotyping of only a couple legume species. Here we describe an in-solution probe capture based method for application of TES in legumes. The topics cover probe design, library preparation, probe hybridization, as well as bioinformatic analysis for evaluation of target capture efficiency and identifying single nucleotide polymorphisms using generated sequencing data.

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Acknowledgments

This work was supported by the Florida Peanut Producers Association and National Peanut Board.

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Authors and Affiliations

  1. Agronomy Department, University of Florida, Gainesville, FL, USA

    Ze Peng, Dev Paudel, Liping Wang, Ziliang Luo, Qian You & Jianping Wang

  2. Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, Gainesville, FL, USA

    Jianping Wang

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  1. Ze Peng
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  2. Dev Paudel
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  3. Liping Wang
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  4. Ziliang Luo
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  5. Qian You
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  6. Jianping Wang
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Correspondence to Jianping Wang .

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Editors and Affiliations

  1. School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India

    Mukesh Jain

  2. Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India

    Rohini Garg

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Peng, Z., Paudel, D., Wang, L., Luo, Z., You, Q., Wang, J. (2020). Methods for Target Enrichment Sequencing via Probe Capture in Legumes. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0235-5_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0234-8

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