Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization Protocol

  • Fernando López-Gallego
  • Jose M. GuisanEmail author
  • Lorena Betancor
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)


In this chapter, we describe different approaches for the utilization of glutaraldehyde in protein immobilization. First, we focus on the covalent attachment of proteins to glutaraldehyde-activated matrixes. We describe conditions for the synthesis of such supports and provide an example of the immobilization and stabilization of a fructosyltransferase. We also describe how glutaraldehyde may be used for the cross-linking of protein–protein aggregates and protein adsorbed onto amino-activated matrixes. In these cases, glutaraldehyde bridges either two lysine groups from different protein molecules or a lysine from the protein structure and an amine group from the support. Examples of cross-linking are given for the immobilization of a d-amino acid oxidase on different amino-activated supports.

Key words

Glutaraldehyde Protein immobilization Cross-linking Protein stabilization 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Fernando López-Gallego
    • 2
    • 3
  • Jose M. Guisan
    • 1
    Email author
  • Lorena Betancor
    • 1
  1. 1.Institute of Catalysis, CSIC, Campus UAM-CantoblancoMadridSpain
  2. 2.Department of BiocatalysisInstitute of Catalysis and Petrochemistry (ICP) CSIC, Campus UAMMadridSpain
  3. 3.Departamento de Química Orgánica, Instituto de Síntesis Química y Catálisis Homogénea (ISQCH) CSIC-Universidad de ZaragozaZaragozaSpain

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