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Immobilization of Cells on Polyurethane Foam

  • Ignacio de Ory
  • Gema Cabrera
  • Martin Ramirez
  • Ana BlandinoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)

Abstract

In this chapter, protocols and details for the immobilization of a model cell onto polyurethane foam carriers are provided in order to facilitate the use of such systems in laboratory or industrial reactors. Polyurethane foam has recently acquired great relevance as a carrier for its good mechanical properties, high porosity, and large adsorption surface. In addition, it has a very low commercial cost. Two different immobilization protocols have been described, differing in the flow regime or the possibilities for the reactor: immobilization in a stirred tank reactor working in a discontinuous regime (by cycles) and immobilization in a packed column working in continuous operation mode. Protocols for carrier sterilization, analytical methodology, and immobilization are described.

Key words

Adsorption Packed column Polyurethane foam Stirred tank 

References

  1. 1.
    Moonmangmee S, Kawabata K, Tanaka S, Toyama H, Adachi O, Matsushita K (2002) A novel polysaccharide involved in the pellicle formation of Acetobacter aceti. J Biosci Bioeng 93(2):192–200CrossRefGoogle Scholar
  2. 2.
    Manohar S, Kim CK, Karegoudar TB (2001) Enhanced degradation of naphthalene by immobilization of Pseudomonas sp. strain NGK1 in polyurethane foam. Appl Microbiol Biotechnol 55(3):311–316CrossRefGoogle Scholar
  3. 3.
    Moe WM, Irvine RL (2001) Polyurethane foam based biofilter media for toluene removal. Water Sci Technol 43(11):35–42CrossRefGoogle Scholar
  4. 4.
    Hori H, Yamashita S, Ishii M, Tanji Y, Unno H (2001) Isolation, characterization and application to off-gas treatment of toluene-degrading bacteria. J Chem Eng 34(9):1120–1126CrossRefGoogle Scholar
  5. 5.
    Moe WM, Irvine RL (2001) Effect of nitrogen limitation on performance of toluene degrading biofilters. Water Res 35(6):1407–1414CrossRefGoogle Scholar
  6. 6.
    Yang C, Suidan MT, Zhu X, Kim BJ (2003) Comparison of a single-layer and multi-layer rotating drum biofilters for VOC removal. Environ Prog 22(2):87–94CrossRefGoogle Scholar
  7. 7.
    Burgess JE, Parsons SA, Stuetz RM (2001) Developments in odour control and waste gas treatment biotechnology: a review. Biotechnol Adv 19:35–63CrossRefGoogle Scholar
  8. 8.
    de Ory I, Romero LE, Cantero D (2004) Optimization of immobilization conditions for vinegar production. Siran, wood chips and polyurethane foam as carriers for Acetobacter aceti. Process Biochem 39:547–555CrossRefGoogle Scholar
  9. 9.
    Holubar P, Plas C, Weiss B, Sasshofer S, Braun R (1994) Hydrocarbon removal with a polyurethane foam bioreactor. Biologische Abgasreinigung 1104:505–s510Google Scholar
  10. 10.
    Armentia H, Webb C (1992) Ferrous sulfate oxidation using Thiobacillus ferrooxidans cells immobilised in polyurethane foam support particles. Appl Microbiol Biotechnol 36:697–700CrossRefGoogle Scholar
  11. 11.
    Mori A (1985) Production of vinegar by immobilized cells. Process Biochem 20(3):67–74Google Scholar
  12. 12.
    Van Loosdrecht MCM, Eikelboom D, Gjaltema A, Mulder A, Tijhuis L, Heijnen JJ (1995) Biofilm structures. Wat Sci Technol 32(8):35–43CrossRefGoogle Scholar
  13. 13.
    de Ory I, Romero LE, Cantero D (2000) Influence of shear stress on immobilization of acetic acid bacteria on polyurethane foam carriers. Mededelingen-Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen. Universiteit Gent 65(3a):235–241Google Scholar
  14. 14.
    O’Reilly AM, Scott JA (1995) Defined coimmobilization of mixed microorganism cultures. Enzym Microb Technol 17:636–646CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Ignacio de Ory
    • 1
  • Gema Cabrera
    • 1
  • Martin Ramirez
    • 1
  • Ana Blandino
    • 1
    Email author
  1. 1.Department of Chemical Engineering and Food Technology, Faculty of SciencesUniversity of CadizCadizSpain

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