Immobilization of Cells on Polyurethane Foam
In this chapter, protocols and details for the immobilization of a model cell onto polyurethane foam carriers are provided in order to facilitate the use of such systems in laboratory or industrial reactors. Polyurethane foam has recently acquired great relevance as a carrier for its good mechanical properties, high porosity, and large adsorption surface. In addition, it has a very low commercial cost. Two different immobilization protocols have been described, differing in the flow regime or the possibilities for the reactor: immobilization in a stirred tank reactor working in a discontinuous regime (by cycles) and immobilization in a packed column working in continuous operation mode. Protocols for carrier sterilization, analytical methodology, and immobilization are described.
Key wordsAdsorption Packed column Polyurethane foam Stirred tank
- 9.Holubar P, Plas C, Weiss B, Sasshofer S, Braun R (1994) Hydrocarbon removal with a polyurethane foam bioreactor. Biologische Abgasreinigung 1104:505–s510Google Scholar
- 11.Mori A (1985) Production of vinegar by immobilized cells. Process Biochem 20(3):67–74Google Scholar
- 13.de Ory I, Romero LE, Cantero D (2000) Influence of shear stress on immobilization of acetic acid bacteria on polyurethane foam carriers. Mededelingen-Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen. Universiteit Gent 65(3a):235–241Google Scholar