Immobilization of Cells on Polyurethane Foam

  • Ignacio de Ory
  • Gema Cabrera
  • Martin Ramirez
  • Ana BlandinoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)


In this chapter, protocols and details for the immobilization of a model cell onto polyurethane foam carriers are provided in order to facilitate the use of such systems in laboratory or industrial reactors. Polyurethane foam has recently acquired great relevance as a carrier for its good mechanical properties, high porosity, and large adsorption surface. In addition, it has a very low commercial cost. Two different immobilization protocols have been described, differing in the flow regime or the possibilities for the reactor: immobilization in a stirred tank reactor working in a discontinuous regime (by cycles) and immobilization in a packed column working in continuous operation mode. Protocols for carrier sterilization, analytical methodology, and immobilization are described.

Key words

Adsorption Packed column Polyurethane foam Stirred tank 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Ignacio de Ory
    • 1
  • Gema Cabrera
    • 1
  • Martin Ramirez
    • 1
  • Ana Blandino
    • 1
    Email author
  1. 1.Department of Chemical Engineering and Food Technology, Faculty of SciencesUniversity of CadizCadizSpain

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