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Immobilization of INS1E Insulin-Producing Cells Within Injectable Alginate Hydrogels

  • Albert Espona-Noguera
  • Jesús Ciriza
  • Alberto Cañibano-Hernández
  • Laura Saenz del BurgoEmail author
  • Jose Luis PedrazEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)

Abstract

Alginate has demonstrated high applicability as a matrix-forming biomaterial for cell immobilization due to its ability to make hydrogels combined with cells in a rapid and non-toxic manner in physiological conditions, while showing excellent biocompatibility, preserving immobilized cell viability and function. Moreover, depending on its application, alginate hydrogel physicochemical properties such as porosity, stiffness, gelation time, and injectability can be tuned. This technology has been applied to several cell types that are able to produce therapeutic factors. In particular, alginate has been the most commonly used material in pancreatic islet entrapment for type 1 diabetes mellitus treatment. This chapter compiles information regarding the alginate handling, and we describe the most important steps and recommendations to immobilize insulin-producing cells within a tuned injectable alginate hydrogel using a syringe-based mixing system, detailing how to assess the viability and the biological functionality of the embedded cells.

Key words

Alginate Hydrogel Tissue engineering Diabetes mellitus Controlled drug delivery system Insulin-producing cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Albert Espona-Noguera
    • 1
    • 2
  • Jesús Ciriza
    • 1
    • 2
  • Alberto Cañibano-Hernández
    • 1
    • 2
  • Laura Saenz del Burgo
    • 1
    • 2
    Email author
  • Jose Luis Pedraz
    • 1
    • 2
    Email author
  1. 1.NanoBioCel Group, Laboratory of PharmaceuticsSchool of Pharmacy, University of the Basque Country (UPV/EHU)Vitoria-GasteizSpain
  2. 2.Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN)Vitoria-GasteizSpain

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