Encapsulation of Enzymes in Porous Capsules via Particle Templating

  • Mikhail V. Zyzin
  • Pedro Ramos-Cabrer
  • Susana Carregal-RomeroEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)


The entrapment of enzymes in capsules is a smart strategy to concentrate them in confined spaces and control their exposure to outside environments. Enzymes can be caged in the interior of capsules during their formation (preloading) or postloaded within prefabricated and permeable hollow shells. On the other hand, enzymes can also be deposited within the shell or on the surface of the capsules. Each of these strategies has intrinsic limitations, and a common enemy is the undesired desorption of enzymes.

Here, we describe the formation of enzyme-loaded polymeric capsules prepared with the Layer-by-Layer method and the template-assisted entrapment of enzymes through coprecipitation (preloading) within calcium carbonate particles, as an example of an efficient preloading strategy, and draw attention at the key parameters that influence this immobilization method.

Key words

Calcium carbonate Layer-by-layer Self-assembly Enzymatic activity Polyelectrolyte capsules 



MVZ acknowledges the President’s Scholarship SP-1576.2018.4 and the Russian Science Foundation for funding (Grant N 19-75-00039).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Mikhail V. Zyzin
    • 1
  • Pedro Ramos-Cabrer
    • 2
    • 3
  • Susana Carregal-Romero
    • 4
    • 5
    Email author
  1. 1.Faculty of Physics and EngineeringITMO UniversitySt. PetersburgRussia
  2. 2.Magnetic Resonance Imaging Lab, CIC biomaGUNEDonostia-San SebastianSpain
  3. 3.Ikerbasque, Basque Foundation for ScienceBilbaoSpain
  4. 4.Molecular and Functional Biomarkers, CIC biomaGUNEDonostia-San SebastianSpain
  5. 5.CIBER Enfermedades Respiratorias (CIBERES)MadridSpain

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