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Immobilization of Enzymes in Protein Films

  • Daniel Sánchez-deAlcázar
  • Mantas Liutkus
  • Aitziber L. CortajarenaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)

Abstract

Heterogeneous biocatalysis usually involves the use of immobilized enzymes on solid supports. Enzymes have suitable properties in terms of efficiency and selectivity for use as immobilized catalysts. Different approaches have been developed for effective immobilization, including adsorption, covalent binding, entrapment, encapsulation, and cross-linking. Those systems offer some advantages with regard to homogeneous catalysts in solution, such as low costs, easy separation and recovery of the catalyst, reusability, and enzymatic stability. Here, we describe a new approach for the immobilization of active enzymes into homogenous films composed solely of scaffolding proteins that differs from the standard methods of enzyme immobilization on solid supports.

Key words

Enzyme Enzyme immobilization Entrapment Protein scaffolds Protein films Protein materials Heterogeneous catalysis 

Notes

Acknowledgments

Financial support for this research was obtained from the Agencia Estatal de Investigació, Spain (BIO2016-77367-R and ERACoBioTech HOMBIOCAT-PCI2018-092984), and the Basque Government (Elkartek KK-2017/00008). This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency– grant no. MDM-2017-0720 (CIC biomaGUNE).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Daniel Sánchez-deAlcázar
    • 1
  • Mantas Liutkus
    • 1
  • Aitziber L. Cortajarena
    • 1
    • 2
    Email author
  1. 1.CIC biomaGUNEDonostia-San SebastianSpain
  2. 2.Ikerbasque, Basque Foundation for ScienceBilbaoSpain

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