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MAIT Cells pp 209-218 | Cite as

Immune Monitoring of Human Mucosal-Associated Invariant T Cells by Quantitative Proteomics

  • Isabel Bernal
  • Marco van Ham
  • Lothar JänschEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Abstract

Molecular phenotypes of mucosal-associated invariant T (MAIT) cells are correlating with individual susceptibilities and outcomes in human diseases. Quantitative proteome strategies can examine such variations in the functional and druggable inventory of MAIT cells comprehensively, but protocols for the support of translational and clinical studies are still rare. Here, we describe a protocol in which MR1-restricted MAIT cells were isolated from blood donations by FACS and are then characterized by quantitative proteomics (iTRAQ-LC-MS/MS) to complement information about their unique effector phenotype and to investigate donor-/patient- or disease-specific variations in protein networks with high precision.

Key words

MR1 tetramer Mucosal-associated invariant T cells Clinical proteomics 8-plex iTRAQ™ 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Isabel Bernal
    • 1
    • 2
    • 3
  • Marco van Ham
    • 1
  • Lothar Jänsch
    • 1
    Email author
  1. 1.Cellular ProteomicsHelmholtz Centre for Infection ResearchBraunschweigGermany
  2. 2.Institute of Medical Microbiology and Hospital Hygiene, Infection ImmunologyOtto-von-Guericke University MagdeburgMagdeburgGermany
  3. 3.ESF Graduate School ABINEPMagdeburgGermany

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