MAIT Cells pp 179-188 | Cite as

A Flow Chamber Assay for Studying MAIT Cell Trafficking

  • Farhat Parween
  • Hongwei H. Zhang
  • Joshua M. FarberEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2098)


Human MAIT cells show little expression of the selectin CD62L and the chemokine receptor CCR7, which are important for entering lymph nodes, and high expression of selectin ligands and chemokine receptors that mediate trafficking into inflamed tissue. Extravasation of leukocytes into tissue requires sequential steps including rolling, firm arrest, crawling, and transendothelial migration, and can be modeled using endothelial cell monolayers in flow chambers that approximate the sheer stress found in post-capillary venules. Using MAIT cells purified from elutriated lymphocytes by fluorescence-activated cell sorting, we have used flow chambers to demonstrate roles for individual chemokine receptors in specific steps required for extravasation. These methods provide a general way to study the molecular mechanisms underlying MAIT cell trafficking from blood into tissue.

Key words

MAIT cells Transendothelial migration Flow chamber Lymphocytes Chemokines 



This research was supported by the Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Farhat Parween
    • 1
  • Hongwei H. Zhang
    • 1
  • Joshua M. Farber
    • 1
    Email author
  1. 1.Inflammation Biology Section, Laboratory of Molecular ImmunologyNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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