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Methods to Visualize Auxin and Cytokinin Signaling Activity in the Shoot Apical Meristem

  • Géraldine Brunoud
  • Carlos S. Galvan-Ampudia
  • Teva VernouxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2094)

Abstract

Visualizing the distribution of hormone signaling activity such as auxin and cytokinins is of key importance for understanding regulation of plant development and physiology. Live imaging and genetically encoded hormone biosensors and reporters allow monitoring the spatial and temporal distribution of these phytohormones. Here, we describe how to cultivate live shoot apical meristems after dissection for observation under the confocal microscope for up to 4 days. The shoot apical meristems are maintained on an appropriate medium allowing them to grow and initiate new organs at a frequency similar to plants grown on soil. Meristems expressing hormone biosensors and reporters allows following hormone signaling activity distribution at high spatiotemporal resolution without chemical fixation, an approach that that can also be applied to follow the dynamics of expression in vivo of any fluorescent marker.

Key words

Shoot apical meristem Phytohormones biosensors and reporters Live imaging Confocal microscopy 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Géraldine Brunoud
    • 1
  • Carlos S. Galvan-Ampudia
    • 1
  • Teva Vernoux
    • 1
    Email author
  1. 1.Laboratoire Reproduction et Développement des Plantes (RDP), Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRALyonFrance

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