Advertisement

Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture Their Transcriptome Profile

  • Monika Mahajan
  • Ram Kishor YadavEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2094)

Abstract

In higher plants, the cells that form aboveground tissues and organs are derived from the shoot apical meristem (SAM). SAM is dynamic in nature and divided into central zone (CZ), peripheral zone (PZ), and rib meristem (RM). Stem cells reside in the CZ, and their progenitors differentiate to form lateral organs in PZ and stem tissue in RM. Besides zones, the SAM is also divided into distinct clonal cell layers that show patterned cell division. Here, we describe methods to tag and isolate cell types from both cell layers and zones of SAM in high purity using cell sorter. This method enable plant biologist in rapid isolation of desired cell types from SAM to record their transcriptome, epigenome, proteome, and metabolome. The information generated by this approach will elucidate the mechanism of stem cell self-renewal, differentiation, and organogenesis in SAM.

Key words

GFP Stem cells FACS Sorting SAM Cell types 

References

  1. 1.
    Steeves Taylor A, Sussex IM (1989) Pattern in plant development. Cambridge University Press, New YorkCrossRefGoogle Scholar
  2. 2.
    Satina S, Blakeslee AF, Avery AG (1940) Demonstration of the three germ layers in the shoot apex of datura by means of induced polyploidy in periclinal chimeras on JSTOR. Am J Bot 27(10):895–905.  https://doi.org/10.2307/2436558CrossRefGoogle Scholar
  3. 3.
    Brady SM, Orlando D, Lee JY, Wang JY, Koch J, Dinneny JR, Mace D, Ohler U, Benfey PN (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318:801–806CrossRefGoogle Scholar
  4. 4.
    Yadav RK, Girke T, Pasala S, Xie M, Reddy GV (2009) Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. Proc Natl Acad Sci U S A 106:4941–4946CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Indian Institute of Science Education and Research MohaliSAS NagarIndia

Personalised recommendations