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A New Perspective on Cryotherapy: Pathogen Elimination Using Plant Shoot Apical Meristem via Cryogenic Techniques

  • Ergun KayaEmail author
  • Selin Galatali
  • Sevinc Guldag
  • Onur Celik
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2094)

Abstract

Plant pathogens cause different diseases on crops and industrial plant species that result in economic losses. Pathogen-free plant material has usually been obtained by traditional procedures such as meristem culture, thermotherapy, and chemotherapy. However, there are many limitations of these procedures such as mechanical challenges of meristem excision and low regeneration rate, low resistance to high temperatures, phytotoxicity, and mutagenic effects of the chemicals used in the procedures. Cryotherapy is a newly developed biotechnological tool that has been very effective in virus elimination from economically important plant species. This tool has overcome the abovementioned limitations. This chapter aims to highlight the importance of the cryogenic procedures (vitrification, encapsulation-vitrification, droplet vitrification, two-step freezing, dehydration, encapsulation-dehydration) in order to generate virus-free germplasm.

Key words

Plant biotechnology Dehydration Liquid nitrogen Plant viruses Vitrification 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Ergun Kaya
    • 1
    Email author
  • Selin Galatali
    • 1
  • Sevinc Guldag
    • 1
  • Onur Celik
    • 1
  1. 1.Molecular Biology and Genetics Department, Faculty of ScienceMugla Sitki Kocman UniversityMuglaTurkey

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