Micropropagation of Rosaceous Species SAM Grown in Temperate Climate

  • Jiri SedlakEmail author
  • Frantisek Paprstein
Part of the Methods in Molecular Biology book series (MIMB, volume 2094)


The benefits of in vitro plant cultivation are mainly due to very high multiplication rate. Cultivation of plant material in vitro can be carried out during the whole year regardless of the time of the year or weather conditions. We create artificial conditions in the lab (heat, light, humidity), and we can regulate these conditions at any time. For the preservation of cultivar identity, we recommend establishing in vitro cultures from shoot tips usually larger than 0.2 mm. In practice, in vitro cultivation of plants uses these growth regulators to achieve organogenesis, for example, root formation, prolonged growth, or multiplication. During each subculture, these cultures are then transferred on a solid agar medium in the form of actively growing multiple shoots with a well-differentiated shoot tip containing meristematic area. Cytokinins are important for cell division and causes branching of plants. Auxins, both endogenous and exogenous, act at as a trigger for the differentiation and formation of root primordia. Morphological characteristics (formation of leaves or callus) and shoot development should be observed during in vitro multiplication and after transfer to ex vitro conditions.

Key words

In vitro Multiplication rate Growth regulator Media Explant 


  1. 1.
    Bell RL, Reed BM (2002) In vitro tissue culture of pear: advances in techniques for micropropagation and germplasm preservation. Acta Hortic 596:412–418CrossRefGoogle Scholar
  2. 2.
    Durkovic J (2006) Rapid micropropagation of mature wild cherry. Biol Plant 50(4):733–736CrossRefGoogle Scholar
  3. 3.
    Erbenova M, Paprstein F, Sedlak J (2001) In vitro propagation of dwarfed rootstocks for sweet cherry. Acta Hortic 560:477–480CrossRefGoogle Scholar
  4. 4.
    Hossini AD, Moghadam EG, Anahid S (2010) Effects of media cultures and plant growth regulators in micro propagation of gisela 6 rootstock. Ann Biol Res 1(2):135–141Google Scholar
  5. 5.
    Mansseri-Lamrioui A, Louerguioui A, Bonaly J, Yakoub-Bougdal S, Allili N, Gana-Kebbouche S (2011) Proliferation and rooting of wild cherry: the influence of cytokinin and auxin types and their concentration. Afr J Biotechnol 10(43):8613–8624CrossRefGoogle Scholar
  6. 6.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  7. 7.
    Reed BM, DeNoma J (2016) Medium-term in vitro storage of pear as a complementary germplasm preservation technique. Acta Hortic 1113:251–256CrossRefGoogle Scholar
  8. 8.
    Ruzic D, Vujovic TI (2008) The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). HortSci 35(1):12–21Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Fruit GenebanksResearch and Breeding Institute of Pomology Holovousy Ltd.HoriceCzech Republic

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