3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox
The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
Key wordsRAM Proliferation Cell cycle Image analysis Arabidopsis Epidermis Cortex Endodermis Pericycle Protophloem Protoxylem
We thank Roland Nitschke and the staff of the Life Imaging Center (LIC) in the Center for Biological Systems Analysis (ZBSA) of the Albert-Ludwigs-University Freiburg for help with their confocal microscopy resources. This work was supported by the Russian State Budget Project (0324-2019-0040), by the Russian Federation President Grant for young scientists (MK-1297.2017.4), and by the Russian Foundation for Basic Research (RFBR-18-34-00485). This work was also supported by Bundesministerium für Bildung und Forschung (BMBF Microsystems, Haploswitch), the German Research Foundation (DFG SFB746, INST 39/839,840,841), and Deutsches Zentrum für Luft und Raumfahrt (DLR 50WB1022). The authors declare that no competing interests exist.
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