Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants
Activity is important for neural development and regeneration. Enhancing neural activity can facilitate axon regrowth of retinal ganglion cells. Here, we describe various methods, including electrical stimulation, pharmacological manipulation, and optogenetics, to elevate neural activity of retinal explants in mice and to analyze their effects on promoting neurite outgrowth in organotypic culture.
Key wordsRetinal ganglion cells Axon regeneration Electrical stimulation Optogenetics Organotypic culture
We thank Dr. Chih-Tien Wang for kindly providing us with the recipe for culturing retinal explants. We also thank Dr. Ron Meyer and Ms. Jill Miotke for providing us with their laboratory protocols for the fixation and immunostaining of retinal explants. We appreciate Mr. Yueh-Chun Tsai in assisting us to assemble the Arduino microcontroller board for controlling blue light stimulation. This project was supported by the Ministry of Science and Technology in Taiwan, MOST-107-2311-B-007-002-MY3 (to C.C.C.).
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