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Retinal Strip Culture for Studying Ganglion Cell Axon Growth

  • Masayuki YamashitaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2092)

Abstract

Retinal ganglion cell (RGC) axons extend along the inner limiting membrane, which forms the extracellular matrix (ECM) containing laminin, collagen, and proteoglycans. RGC axons express integrin, which is activated by binding to ECM proteins to regulate cytoskeleton. To study the growth of RGC axons in vitro, maintaining the natural environment for them is absolutely necessary. For this purpose, culturing a strip of embryonic chick retina in Matrigel® is a suitable method. This article describes detailed techniques of the retinal strip culture.

Key words

Retinal strip Retinal ganglion cell Chick embryo Axon growth Extracellular matrix Axon guidance 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Center for Basic Medical ResearchInternational University of Health and WelfareOhtawaraJapan

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