Retinal ganglion cell (RGC) axons extend along the inner limiting membrane, which forms the extracellular matrix (ECM) containing laminin, collagen, and proteoglycans. RGC axons express integrin, which is activated by binding to ECM proteins to regulate cytoskeleton. To study the growth of RGC axons in vitro, maintaining the natural environment for them is absolutely necessary. For this purpose, culturing a strip of embryonic chick retina in Matrigel® is a suitable method. This article describes detailed techniques of the retinal strip culture.
Srichai MB, Zent R (2010) Integrin structure and function. In: Zent R, Pozzi A (eds) Cell-extracellular matrix interactions in cancer. Springer, BerlinGoogle Scholar
Kim S-H, Turnbull J, Guimond S (2011) Extracellular matrix and cell signalling: the dynamic cooperation of integrin, proteoglycan and growth factor receptor. J Endocrinol 209:139–151CrossRefGoogle Scholar
Yamashita M (2015) Weak electric fields serve as guidance cues that direct retinal ganglion cell axons in vitro. Biochem Biophys Rep 4:83–88PubMedPubMedCentralGoogle Scholar