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Intracellular Dye Microinjection in Retinal Morphological and Communication Studies

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2092)

Abstract

The retina provides an excellent system to analyze neural architecture and communication mechanisms. In retinal physiological, morphological, and developmental studies, intracellular dye microinjection is an extensively applied technique. Dyes and/or chemical compounds are introduced into retinal neurons via iontophoresis injection to identify cell types, communication, and developmental changes. Here, we describe the detailed methods for ejecting the dyes Lucifer yellow and Neurobiotin into retinal neurons through micropipette and concentrate on avoiding the known pitfalls and troubleshooting.

Key words

Intracellular microinjection Cell identification Gap junctions Neural communication Lucifer yellow Neurobiotin Micropipette Iontophoresis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Ruiz Department of Ophthalmology and Visual ScienceMcGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth)HoustonUSA

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