Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells

  • Andrew T. Hale
  • Bradley P. Clarke
  • John D. YorkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2091)


Inositol phosphate (IP) and phosphatidylinositol (PI) signaling are critical signal transduction pathways responsible for generating numerous receptor-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling, but detailed characterization of unique IP and PI signaling profiles in response to different agonists and among cell types remains largely unexplored. Here, we outline steady-state inositol metabolic-labeling techniques that can be leveraged to assess the IP and PI signaling state in eukaryotic cells. This flexible technique can be amended and optimized to your cell line of interest, perturbed with biochemical, genetic, or pharmacological alteration, and used to provide comprehensive inositol profiling in various cellular systems.

Key words

Inositol phosphates Phosphatidylinositol Metabolic labeling Signal transduction HPLC Lipid signaling 



This work was supported by funds from the National Institutes of Health (5T32GM007347, A.T.H.) and (1R01GM12440401, J.D.Y).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Andrew T. Hale
    • 1
  • Bradley P. Clarke
    • 1
  • John D. York
    • 1
    Email author
  1. 1.Department of BiochemistryVanderbilt University School of MedicineNashvilleUSA

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