Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation

  • Benjamin L. TurnerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2091)


Inositol hexakisphosphates are extracted from soil in strong alkali and isolated from other organic phosphates by hypobromite oxidation. The procedure yields the four stereoisomeric forms of inositol hexakisphosphate in a form suitable for spectroscopic or chromatographic identification.

Key words

Inositol phosphate NMR spectroscopy Phytate Phytic acid Soil Sediment Stereoisomers 


  1. 1.
    Cosgrove DJ (1980) Inositol phosphates - their chemistry, biochemistry and physiology. Elsevier Scientific, AmsterdamGoogle Scholar
  2. 2.
    Turner BL (2007) Inositol phosphates in soil: amounts, forms and significance of the phosphorlyated inositol stereoisomers. In: Turner BL, Richardson AE, Mullaney EJ (eds) Inositol phosphates: linking agriculture and the environment. CAB International, Wallingford, pp 186–207CrossRefGoogle Scholar
  3. 3.
    Raboy V (2003) myo-Inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry 64:1033–1043CrossRefGoogle Scholar
  4. 4.
    Turner BL, Cheesman AW, Godage HY, Riley AM, Potter BVL (2012) Determination of neo- and D-chiro-inositol hexakisphosphate in soils by solution 31P NMR spectroscopy. Environ Sci Technol 46:4994–5002. Scholar
  5. 5.
    Turner BL, Richardson AE (2004) Identification of scyllo-inositol phosphates in soils by solution phosphorus-31 nuclear magnetic resonance spectroscopy. Soil Sci Soc Am J 68(3):802–808CrossRefGoogle Scholar
  6. 6.
    Cade-Menun BJ, Preston CM (1996) A comparison of soil extraction procedures for 31P NMR spectroscopy. Soil Sci 161(11):770–785CrossRefGoogle Scholar
  7. 7.
    Wrenshall CL, Dyer WJ (1941) Organic phosphorus in soils: II. The nature of the organic phosphorus compounds. A. Nucleic acid derivatives. B. Phytin. Soil Sci 51:235–248CrossRefGoogle Scholar
  8. 8.
    Irving GCJ, Cosgrove DJ (1981) The use of hypobromite oxidation to evaluate two current methods for the estimation of inositol polyphosphates in alkaline extracts of soils. Commun Soil Sci Plant Anal 12(5):495–509CrossRefGoogle Scholar
  9. 9.
    Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36CrossRefGoogle Scholar
  10. 10.
    Bowman RA, Moir JO (1993) Basic EDTA as an extractant for soil organic phosphorus. Soil Sci Soc Am J 57:1516–1518CrossRefGoogle Scholar
  11. 11.
    Turner BL, Cade-Menun BJ, Condron LM, Newman S (2005) Extraction of soil organic phosphorus. Talanta 66(2):294–306CrossRefGoogle Scholar
  12. 12.
    Turner BL, Mahieu N, Condron LM (2003) The phosphorus composition of temperate pasture soils determined by NaOH-EDTA extraction and solution 31P NMR spectroscopy. Org Geochem 34(8):1199–1210CrossRefGoogle Scholar
  13. 13.
    Rowland AP, Haygarth PM (1997) Determination of total dissolved phosphorus in soil solutions. J Environ Qual 26:410–415CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Smithsonian Tropical Research InstituteBalboaRepublic of Panama

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