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Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation

  • Benjamin L. TurnerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2091)

Abstract

Inositol hexakisphosphates are extracted from soil in strong alkali and isolated from other organic phosphates by hypobromite oxidation. The procedure yields the four stereoisomeric forms of inositol hexakisphosphate in a form suitable for spectroscopic or chromatographic identification.

Key words

Inositol phosphate NMR spectroscopy Phytate Phytic acid Soil Sediment Stereoisomers 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Smithsonian Tropical Research InstituteBalboaRepublic of Panama

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