Abstract
Optical molecular and cellular imaging has provided considerable information in various life science research fields. In particular, pHluorin-based assays allow us to specifically investigate presynaptic physiological processes, such as synaptic transmission and synaptic retrieval. Here, I describe quantitative synaptic transmission in primary cultured hippocampal neurons using a vGlut-pHluorin system combined with a high-fidelity optical imaging system and its regulation by inositol pyrophosphate.
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Acknowledgments
This work was supported by National Research Foundation of Korea Grant 2017R1A2B4007019 funded by the Ministry of Science and Information and Communication Technology.
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Kim, S.H. (2020). Optical Measurement of Synaptic Vesicle Fusion and Its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons. In: Miller, G. (eds) Inositol Phosphates. Methods in Molecular Biology, vol 2091. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0167-9_11
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DOI: https://doi.org/10.1007/978-1-0716-0167-9_11
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