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Lipoxygenases as Targets for Drug Development

  • Xiao-Yuan MaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2089)

Abstract

Lipoxygenases are key enzymes that catalyze the polyunsaturated fatty acids such as arachidic acid, linoleic acid (LA), and others unsaturated fatty acids. They are involved in important functions such as cell structure, metabolism, and signal transduction mechanisms, finally mediating cell death process, especially ferroptosis, a novel type of cell death modality. Our present protocol described a colorimetric assay for measuring lipoxygenase activity as well as a high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method for the quantification of arachidonic acid metabolites.

Key words

Lipoxygenases Arachidic acid Leukotrienes Polyunsaturated fatty acids 

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Copyright information

© Springer Science+Business Media, LLC 2020

Authors and Affiliations

  1. 1.Department of Clinical Pharmacology, Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China
  2. 2.Institute of Clinical PharmacologyCentral South University, Hunan Key Laboratory of PharmacogeneticsChangshaPeople’s Republic of China
  3. 3.Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of EducationChangshaPeople’s Republic of China
  4. 4.National Clinical Research Center for Geriatric DisordersChangshaPeople’s Republic of China

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