Neutrophil pp 277-298 | Cite as

Genome-Scale Transcript Analyses of Human Neutrophils

  • Scott D. Kobayashi
  • Adeline R. Porter
  • Sarah L. Anzick
  • Dan E. Sturdevant
  • Frank R. DeLeoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2087)


Transcriptome analyses of unicellular and multicellular organisms have changed fundamental understanding of biological and pathological processes across multiple scientific disciplines. Over the past 15 years, studies of polymorphonuclear leukocyte (PMN or neutrophil) gene expression on a global scale have provided new insight into the molecular processes that promote resolution of infections in humans. Herein we present methods to analyze gene expression in human neutrophils using Affymetrix oligonucleotide microarrays and next-generation sequencing. Notably, the procedures utilize commercially available reagents and materials and thus represent a standardized approach for evaluating PMN transcript levels.

Key words

Neutrophil Microarray Gene expression Affymetrix Next-generation sequencing Transcript Phagocyte Polymorphonuclear leukocytes 



This work was supported by the Intramural Program of the National Institutes of Allergy and Infectious Diseases, NIH.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Scott D. Kobayashi
    • 1
  • Adeline R. Porter
    • 1
  • Sarah L. Anzick
    • 2
  • Dan E. Sturdevant
    • 2
  • Frank R. DeLeo
    • 3
    Email author
  1. 1.Laboratory of Bacteriology, Rocky Mountain LaboratoriesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA
  2. 2.Genomics Unit, Rocky Mountain LaboratoriesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA
  3. 3.Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural ResearchNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA

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