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Neutrophil pp 191-205 | Cite as

Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils

  • Maurice B. HallettEmail author
  • Rhiannon E. Roberts
  • Sharon Dewitt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2087)

Abstract

The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils, and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

Key words

Calcium signaling Cytosolic calcium flux Fluorescent calcium indicator dye Photoactivation Calcium imaging 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Maurice B. Hallett
    • 1
    Email author
  • Rhiannon E. Roberts
    • 1
  • Sharon Dewitt
    • 2
  1. 1.Neutrophil Signalling GroupCardiffUK
  2. 2.School of Medicine and School of DentistryCardiff UniversityCardiffUK

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