Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays

  • Renata Nacasaki SilvestreEmail author
  • Pablo Diego Moço
  • Virgínia Picanço-Castro
Part of the Methods in Molecular Biology book series (MIMB, volume 2086)


Immunotherapy using T cells modified with chimeric antigen receptor (CAR) has been proven effective in the treatment of leukemia and lymphomas resistant to chemotherapy. Recent clinical studies have shown excellent responses of CAR-T cells in a variety of B cell tumors. However, it is important to validate in vitro activity of these cells, though different sorts of assays, which are capable of measuring the cytotoxic potential of these cells. In this chapter, it will be pointed two methods to evaluate CAR-T cell killing potential against B cell malignancy cell lines.

Key words

Chimeric antigen receptor Cytotoxicity Human cell line B cell malignancies 



The authors acknowledge São Paulo Research Foundation—FAPESP (2015/19017-6, 2016/08374-5), the National Council for Scientific and Technological Development—CNPq (142406/2016-3), Research, Innovation, and Dissemination Centers—RIDC (2013/08135-2), the Coordination of Improvement of Higher Level Personnel – Capes (88887.140966/2017-00) and the National Institute of Science and Technology in Stem Cell and Cell Therapy—INCTC (465539/2014-9) for financial support. The authors also acknowledge financial support from Secretaria Executiva do Ministério da Saúde (SE/MS), Departamento de Economia da Saúde, Investimentos e Desenvolvimento (DESID/SE), Programa Nacional de Apoio à Atenção Oncológica (PRONON) Process 25000.189625/2016-16.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Renata Nacasaki Silvestre
    • 1
    Email author
  • Pablo Diego Moço
    • 1
  • Virgínia Picanço-Castro
    • 1
  1. 1.Center for Cell-Based Therapy CTC, Regional Blood Center of Ribeirão PretoUniversity of São PauloSão PauloBrazil

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