Immunophenotypic Analysis of CAR-T Cells

  • Júlia Teixeira Cottas de AzevedoEmail author
  • Amanda Mizukami
  • Pablo Diego Moço
  • Kelen Cristina Ribeiro Malmegrim
Part of the Methods in Molecular Biology book series (MIMB, volume 2086)


CAR-T cell immunotherapy is a promising therapeutic modality for cancer patients. The success of CAR-T cell therapy has been associated with the phenotype, activation and functional profiling of infused CAR-T cells. Therefore, immunophenotypic characterization of CAR-T cells during bioprocess is crucial for cell quality control and ultimately for improved antitumor efficacy. In this chapter, we propose a flow cytometry panel to characterize the immunophenotype of the CAR-T subsets.

Key words

CAR-T cells Immunophenotypic analysis Flow cytometry T cells subsets Naive T cell Central memory T cell Effector memory T cell Effector T cell Memory stem T cells 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Júlia Teixeira Cottas de Azevedo
    • 1
    • 2
    • 3
    Email author
  • Amanda Mizukami
    • 1
  • Pablo Diego Moço
    • 1
  • Kelen Cristina Ribeiro Malmegrim
    • 4
  1. 1.Center for Cell-based Therapy, Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  2. 2.Department of Biochemistry and Immunology, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  3. 3.Department of Diabetes Immunology, Diabetes and Metabolism Research Institute at the Beckman Research Institute of City of Hope, DuarteCAUSA
  4. 4.School of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloSão PauloBrazil

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