CAR-T Cell Expansion in a Xuri Cell Expansion System W25
Cell expansion is typically a long and labor-intensive step in CAR-T cell manufacture. The Xuri Cell Expansion System (CES) W25 semiautomates this step while functionally closing the process. Cells for autologous or allogeneic cell therapies are cultured inside a single-use Xuri Cellbag™ bioreactor. Wave-induced agitation, performed by a rocking Base Unit, transfers gas and mixes the culture. The integral UNICORN™ software allows customization of culture conditions and media perfusion schedules. Culture volumes can range from 300 mL to 25 L, making the Xuri CES W25 system suitable for both scale-up and scale-out manufacturing processes. CAR-T cell therapies have been successfully generated using the Xuri CES W25 system, which reduces manual labor compared with static culturing methods. This chapter details how to initiate a culture, install the Xuri CES W25, and install a 2 L Cellbag bioreactor. Protocols on inoculation, monitoring, and sampling are also outlined in this chapter.
Key wordsT lymphocytes CAR-T cells Immunotherapy Cell therapy Gene therapy Bioreactor Autologous Allogeneic
The author thanks Matt Sherman, PE for providing the 2 L Cellbag bioreactor line drawing in Fig. 2, the Cellular Biology team at the GE Global Research Center for their continued mentorship, and the Centre for Commercialization of Regenerative Medicine for their persistence in expanding the applications of the Xuri Cell Expansion System W25.
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