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Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry

  • Yingyao Liu
  • Rajesh N. PatkarEmail author
  • Naweed I. NaqviEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2085)

Abstract

Liquid chromatography–mass spectrometry (LC-MS) is one of the most important analytical chemistry techniques for the detection and characterization of biologically active compounds of low abundance—for example, hormones. Gas chromatography (GC) coupled with mass spectrometry has been a method of choice to detect jasmonic acid, the well-known defense hormone in plants. Recently, we identified structural and functional analogs of phytohormone jasmonic acid (JA) and its derivatives, in the rice-blast fungus Magnaporthe oryzae. Here, we describe protocols of LC-MS/MS-based identification and quantification of fungal jasmonates, especially during pathogenic development in the rice blast fungus.

Key words

Liquid chromatography Small molecule Mass spectrometry Jasmonates Oxylipin Secondary metabolites Fungal pathogen 

Notes

Acknowledgments

We thank the Temasek Life Sciences Laboratory (TLL Singapore) and the National Research Foundation (PMO Singapore, NRF-CRP7-2010-02 and NRF-CRP16-2015-04) for financial support. RP is supported by the DBT-Ramalingaswami Fellowship (BT/RLF/Re-entry/32/2014; Department of Biotechnology, Government of India).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Temasek Life Sciences LaboratorySingaporeSingapore
  2. 2.Bharat Chattoo Genome Research Centre, Department of Microbiology and Biotechnology CentreThe Maharaja Sayajirao University of BarodaVadodaraIndia

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