Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry
Liquid chromatography–mass spectrometry (LC-MS) is one of the most important analytical chemistry techniques for the detection and characterization of biologically active compounds of low abundance—for example, hormones. Gas chromatography (GC) coupled with mass spectrometry has been a method of choice to detect jasmonic acid, the well-known defense hormone in plants. Recently, we identified structural and functional analogs of phytohormone jasmonic acid (JA) and its derivatives, in the rice-blast fungus Magnaporthe oryzae. Here, we describe protocols of LC-MS/MS-based identification and quantification of fungal jasmonates, especially during pathogenic development in the rice blast fungus.
Key wordsLiquid chromatography Small molecule Mass spectrometry Jasmonates Oxylipin Secondary metabolites Fungal pathogen
We thank the Temasek Life Sciences Laboratory (TLL Singapore) and the National Research Foundation (PMO Singapore, NRF-CRP7-2010-02 and NRF-CRP16-2015-04) for financial support. RP is supported by the DBT-Ramalingaswami Fellowship (BT/RLF/Re-entry/32/2014; Department of Biotechnology, Government of India).
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