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Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses

  • Anna S. Dolgova
  • Marina V. Safonova
  • Vladimir G. Dedkov
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2063)

Abstract

This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.

Key words

Efficient sequencing protocols dsRNA viruses Enzyme treatment Precipitation by LiCl FL amplification 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Anna S. Dolgova
    • 1
  • Marina V. Safonova
    • 2
  • Vladimir G. Dedkov
    • 1
    • 3
  1. 1.Saint-Petersburg Pasteur Institute, CenterFederal Service on Consumers’ Rights Protection and Human Well-Being SurveillanceSaint-PetersburgRussia
  2. 2.Plague Control CenterFederal Service on Consumers’ Rights Protection and Human Well-Being SurveillanceMoscowRussia
  3. 3.Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne DiseasesSechenov First Moscow State Medical UniversityMoscowRussia

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