Colorimetry and Spectrophotometer (Spectrophotometry)

  • Mahin Basha
Part of the Springer Protocols Handbooks book series (SPH)


Spectroscopic techniques employ light to interact with matter and thus probe certain features of a sample to learn about its consistency or structure. Light is electromagnetic radiation, a phenomenon exhibiting different energies, and dependent on that energy, different molecular features can be probed. The basic principles of interaction of electromagnetic radiation with matter are treated in this chapter. There is no obvious logical dividing point to split the applications of electromagnetic radiation into parts treated separately. The justification for the split presented in this text is purely pragmatic and based on “common practice.” The applications considered in this chapter use visible or UV light to probe consistency and conformational structure of biological molecules.

Key words

Colorimetry Spectroscopy UV–Vis spectroscopy Beer’s law 





Standard operating procedure


  1. 1.
    Rajan K (2011) Analytical techniques in biochemistry and molecular biology. Springer, New York. eBook ISBN978-1-4419-9785-2Google Scholar
  2. 2.
    Wilson K, Walker J (2010) Principles and techniques of biochemistry and molecular biology. Cambridge University Press, Cambridge. ISBN 978-0-521-73167-6CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Mahin Basha
    • 1
  1. 1.P. M. Sayeed Calicut University CentreUniversity of CalicutLakshadweepIndia

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