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The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

  • Nicholas B. VeraEmail author
  • Michelle Clasquin
  • Stephen L. Coy
  • Paul Vouros
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2084)

Abstract

Differential mobility spectrometry (DMS) is capable of separating molecules based on their size and shape. When coupled to mass spectrometry (MS), DMS reduces chemical background and enhances signal-to-noise (S/N) ratio. Flow injection analysis (FIA) is a technique used to introduce samples into the source of the DMS-MS platform. Here we describe the application of FIA-DMS-MS/MS for the analysis of urinary acylcarnitine species. More than 20 acylcarnitine species can be detected and quantified during a single FIA-DMS-MS/MS acquisition.

Key words

Differential mobility spectrometry Mass spectrometry Acylcarnitine Solid-phase extraction 

Notes

Acknowledgments

This work was funded by the Internal Medicine Research Unit (IMRU), within the Pfizer Global Research and Development Business Unit, at Pfizer.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Nicholas B. Vera
    • 1
    • 2
    Email author
  • Michelle Clasquin
    • 1
  • Stephen L. Coy
    • 2
  • Paul Vouros
    • 2
  1. 1.Cambridge Laboratories, Pfizer Global Research and DevelopmentPfizer Inc.CambridgeUSA
  2. 2.Department of Chemistry and Chemical BiologyNortheastern UniversityBostonUSA

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