Ion Mobility-Mass Spectrometry of Glycoconjugates

  • Weston B. StruweEmail author
  • David J. Harvey
Part of the Methods in Molecular Biology book series (MIMB, volume 2084)


Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes. Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology. Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures. In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.

Key words

Glycosylation Mass spectrometry Ion mobility Glycomics Collision cross section 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Chemistry Research Laboratory, Department of ChemistryUniversity of OxfordOxfordUK
  2. 2.Department of Biochemistry, Oxford Glycobiology InstituteUniversity of OxfordOxfordUK
  3. 3.Nuffield Department of Medicine, Target Discovery InstituteUniversity of OxfordOxfordUK

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