Hydrocarbon and Lipid Microbiology Protocols pp 93-128 | Cite as
Protocols for GC-Based Methods for Generic and Environmental Hydrocarbon Analysis
Abstract
Gas chromatography (GC) is the most widely used analysis for determining the structural distribution and quantification of hydrocarbons in complex mixtures. A sample dissolved in a solvent is vaporized and carried by a gas phase, usually helium or hydrogen, allowing individual components to separate as they interact with a thin-film coating of stationary phase, typically a chemically bonded polymer attached to the inner walls of a capillary column. Identification and quantification of the eluting components are made using universal and/or selective detectors (e.g., flame ionization, mass spectrometer). Several standard industry and government-issued environmental methods are listed, and specific protocols for various hydrocarbon analyses are discussed.
Keywords:
Capillary column FID Gas chromatography Injector MSReferences
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