Protocols for Monitoring Growth and Lipid Accumulation in Oleaginous Yeasts

  • Jean-Marc NicaudEmail author
  • Anne-Marie Crutz-Le Coq
  • Tristan Rossignol
  • Nicolas Morin
Part of the Springer Protocols Handbooks book series (SPH)


Oleaginous yeasts can synthesize and store lipids up to 20% of their dry weight and have emerged as resources of choice for biotechnological applications, such as bio-lipid production. The number of species and mutant libraries consequently available for screening is exponentially growing. Cultivation strategies and growth media for bio-lipid production need to be optimized to accelerate screening and identification of production strains. In this chapter we describe methods for high-throughput cell growth in 96 microtiter plates in various media including opaque broth by using a fluorescent reporter, carbon/nitrogen ratio determination for optimal lipid accumulation, and in vivo real-time detection of lipid accumulation using a neutral lipid fluorescent dye. We provide examples using two well-established oleaginous yeasts, Yarrowia lipolytica and Rhodosporidium toruloides. These methods can be extended to other oleaginous yeast species for high-throughput screening of bio-lipid accumulation.


Lipid accumulation Oleaginous yeast Rhodosporidium Single cell oil Yarrowia 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jean-Marc Nicaud
    • 2
    • 1
    Email author
  • Anne-Marie Crutz-Le Coq
    • 2
    • 1
  • Tristan Rossignol
    • 2
    • 1
  • Nicolas Morin
    • 2
    • 1
  1. 1.AgroParisTechJouy-en-JosasFrance
  2. 2.INRAJouy-en-JosasFrance

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