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Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology

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Induced Pluripotent Stem (iPS) Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2454))

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Abstract

Cardiovascular diseases are one of the leading causes of mortality in the western world. Myocardial infarction is among the most prevalent and results in significant cell loss within the myocardium. Similarly, numerous drugs have been identified as having cardiotoxic side effects. The adult human heart is however unable to instigate an effective repair mechanism and regenerate the myocardium in response to such damage. This is in large part due to the withdrawal of cardiomyocytes (CMs) from the cell cycle. Thus, identifying, screening, and developing agents that could enhance the proliferative capacity of CMs holds great potential in cardiac regeneration. Human induced pluripotent stem cells (hiPSCs) and their cardiovascular derivatives are excellent tools in the search for such agents. This chapter outlines state-of-the art techniques for the two-dimensional differentiation and attainment of hiPSC-derived CMs and endothelial cells (ECs). Bioreactor systems and three-dimensional spheroids derived from hiPSC-cardiovascular derivatives are explored as platforms for drug discovery before focusing on relevant assays that can be employed to assess cell proliferation and viability.

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Acknowledgments

The authors thank Ms. S. Tuuli Karhu for the expert advice on the doxorubicin model. The research was co-funded by the Hungarian National Research, Development and Innovation Fund (NKFIA; NVKP-16-1- 2016-0017, “National Heart Program” and 128444), the NIHR Imperial Biomedical Research Centre (BRC), the Medical Research Council (MR/R025002/1), Academy of Finland (grants 321564, 328909), the Finnish Foundation for Cardiovascular Research, Sigrid Jusélius Foundation, University of Helsinki and Drug Research Doctoral Programme.

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Author notes

  1. Gábor Földes and Virpi Talman contributed equally to this work.

Authors and Affiliations

  1. Drug Research Program and Division of Pharmacology and Pharmacotherapy, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland

    Qasim A. Majid, Lotta Pohjolainen & Virpi Talman

  2. National Heart and Lung Institute, Imperial College London, London, UK

    Qasim A. Majid & Gábor Földes

  3. Heart and Vascular Center, Semmelweis University, Budapest, Hungary

    Barbara Orsolits, Zsófia Kovács & Gábor Földes

Authors
  1. Qasim A. Majid
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  2. Barbara Orsolits
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  3. Lotta Pohjolainen
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  4. Zsófia Kovács
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  5. Gábor Földes
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  6. Virpi Talman
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Corresponding authors

Correspondence to Gábor Földes or Virpi Talman .

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Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Andras Nagy

  2. Ottawa, ON, Canada

    Kursad Turksen

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Majid, Q.A., Orsolits, B., Pohjolainen, L., Kovács, Z., Földes, G., Talman, V. (2021). Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_369

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  • DOI: https://doi.org/10.1007/7651_2021_369

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2118-9

  • Online ISBN: 978-1-0716-2119-6

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