Advertisement

pp 1-3 | Cite as

Dermal-Epidermal Separation by Chemical

  • Liyan Jian
  • Yu Cao
  • Ying Zou
Protocol
Part of the Methods in Molecular Biology book series

Abstract

The skin contains three primary layers: epidermis, dermis, and hypodermis. Separation of epidermal components from dermis (dermal-epidermal separation) is an important basic investigation technique for pharmacology, toxicology, and biology. There are different systems of epidermal separation, including typical methods of chemical, enzyme, heat, etc. Each approach has advantages versus disadvantages, and thus the appropriate method should be chosen for a given research question. Here we described the method of chemical separation.

Keywords

Epidermis Dermis Separation Chemical Sodium thiocyanate 

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China (NSFC) grant No. 81602757, Science and Technology Commission of Shanghai Municipality grant No. 16411961800, and the Science Foundation of Shanghai Hospital Development Center grant No. 16CR3096B.

References

  1. 1.
    Zou Y, Maibach HI (2018) Dermal-epidermal separation methods: research implications. Arch Dermatol Res 310:1–9Google Scholar
  2. 2.
    Diaz LA, Heaphy MR, Calvanico NJ et al (1977) Separation of epidermis from dermis with sodium thiocyanate. J Invest Dermatol 68:36–38Google Scholar
  3. 3.
    Trost A, Bauer JW, Lanschützer C et al (2007) Rapid, high-quality and epidermal-specific isolation of RNA from human skin. Exp Dermatol 16:185–190Google Scholar
  4. 4.
    Felsher Z (1947) Studies on the adherence of the epidermis to the corium. J Invest Dermatol 8:35–47Google Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Liyan Jian
    • 1
  • Yu Cao
    • 1
  • Ying Zou
    • 2
  1. 1.Institute of Precision Medicine, The Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Skin & Cosmetic Research DepartmentShanghai Dermatology HospitalShanghaiChina

Personalised recommendations