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Dermal-Epidermal Separation by Chemical
The skin contains three primary layers: epidermis, dermis, and hypodermis. Separation of epidermal components from dermis (dermal-epidermal separation) is an important basic investigation technique for pharmacology, toxicology, and biology. There are different systems of epidermal separation, including typical methods of chemical, enzyme, heat, etc. Each approach has advantages versus disadvantages, and thus the appropriate method should be chosen for a given research question. Here we described the method of chemical separation.
KeywordsEpidermis Dermis Separation Chemical Sodium thiocyanate
This work was supported by National Natural Science Foundation of China (NSFC) grant No. 81602757, Science and Technology Commission of Shanghai Municipality grant No. 16411961800, and the Science Foundation of Shanghai Hospital Development Center grant No. 16CR3096B.
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