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pp 1-5 | Cite as

Scratch Wound Healing Assay

  • Simona Martinotti
  • Elia RanzatoEmail author
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.

Keywords

Cell migration Data analysis Healing assay Scratch wound assay Wound repair 

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.DiSIT-Dipartimento di Scienze e Innovazione TecnologicaUniversity of Piemonte OrientaleVercelliItaly

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