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Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Electrospun Nanofibers

  • Hasham S. Sofi
  • Nisar Ahmad Khan
  • Faheem A. SheikhEmail author
Protocol
Part of the Methods in Molecular Biology book series

Abstract

An efficient method for the development of myogenic differentiation using the stem cells can be beneficial in patients with severely compromised mobility, muscular damage, or degenerative diseases. The stem cells can prove to be excellent clinical source of myogenic progenitor cells due to their ability of self-proliferation, renewal, and differentiation into a specific phenotype. They represent an essential component of tissue engineering along with other factors (e.g., 3D scaffolds, ECM mimicking environment, and growth factors). In this chapter, we describe the experimental protocols for isolation of the embryonic stem cells, their proliferation on nanofiber scaffolds, and finally their differentiation into myogenic cells. Furthermore, this chapter elaborates experimental methods to assess the myogenic fate of embryonic stem cells on the nanofiber scaffolds.

Keywords

Extracellular substrate Myogenic differentiation Nanofibers Wnt3a protein 

Notes

Acknowledgments

Dr. Faheem A Sheikh acknowledges the financial support received from the Nano Mission Council, Department of Science and Technology, under grant number SR/NM/NM-1038/2016.

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Hasham S. Sofi
    • 1
    • 2
  • Nisar Ahmad Khan
    • 2
  • Faheem A. Sheikh
    • 2
    Email author
  1. 1.Department of NanotechnologyUniversity of KashmirSrinagarIndia
  2. 2.Department of Pharmaceutical SciencesUniversity of KashmirSrinagarIndia

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