Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

  • Aishwarya Sridharan
  • S. Y. John Lim
  • Graham D. Wright
  • Leah A. VardyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2109)


The study of skin pigmentation requires determining the rate of melanin production in melanocytes and quantifying the rate of melanosome transfer to keratinocytes. Here, we describe a method to quantify melanosome transfer using immunofluorescence microscopy coupled with automated image analysis of in vitro human melanocytes and keratinocytes in co-culture. In this method, the number of melanin capped keratinocyte nuclei is quantified.


Melanin transfer quantitation Melanosome transfer Melanocyte keratinocyte co-culture Image quantification 

Supplementary material (6 kb)


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Aishwarya Sridharan
    • 1
  • S. Y. John Lim
    • 1
  • Graham D. Wright
    • 1
  • Leah A. Vardy
    • 1
    • 2
    Email author
  1. 1.Skin Research Institute of Singapore, A*STARSingaporeSingapore
  2. 2.School of Biological SciencesNanyang Technological UniversitySingaporeSingapore

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