pp 1-10 | Cite as

Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope

  • Yueh-Feng Wu
  • Hsin-Yuan Tan
  • Sung-Jan LinEmail author
Part of the Methods in Molecular Biology book series


Multiphoton microscopy allows long-term direct visualization of cells in live animals due to its low photodamage. When coupled with fluorescence protein targeting and second harmonic generation signals from natural collagen as contrast, multiphoton microscopy enables intravital tracing of cells while providing structural information from the extracellular matrix. Compared with conventional histological analysis, it can bring new insight into the cell dynamics in stem cell research. Here, we demonstrate cell imaging and tracing at a single cell resolution in the cornea, skin, and hair follicles using multiphoton microscopy in transgenic mice of which specific cell populations are tagged with fluorescent proteins.


Cornea Skin Hair follicle Multiphoton microscope (MPM) Intravital imaging 



This work was supported by Taiwan Bio-Development Foundation (TBF; to S.J Lin), Taiwan Ministry of Science and Technology (106-2627-M-002-034 to S.J Lin), Taiwan National Health Research Institutes (NHRI-EX107-10410EI to S.J Lin), Chang Gung Memorial Hospital (CMRPG3G1621 and CMRPG3D1691 to H.Y Tan), and Taiwan Ministry of Science and Technology (107-2314-B-182A-089 to H.Y Tan).


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of OphthalmologyChang Gung Memorial HospitalLinkouTaiwan
  3. 3.College of MedicineChang Gung UniversityTaoyuanTaiwan
  4. 4.Department of DermatologyNational Taiwan University Hospital and College of MedicineTaipeiTaiwan
  5. 5.Research Center for Developmental Biology and Regenerative MedicineNational Taiwan UniversityTaipeiTaiwan

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